Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Primer sequences used for reverse transcription polymerase chain reaction analysis of NELIN.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Primer sequences used for reverse transcription polymerase chain reaction analysis of SM22α.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Reverse transcription polymerase chain reaction analysis revealed weak mRNA expression of NELIN in the vascular smooth muscle cells (VSMCs) of the experimental group, but strong expression in the control group. (A) Expected length of the PCR products was 611 bp (NELIN) and 312 bp (β-actin). Lanes: N, control group; X, DNA marker DL 2000; and P, experimental group. (B) Downregulation of NELIN mRNA expression in the VSMCs from the varicose vein tissue. Data are presented as the mean ± standard deviation.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Expression and significance of NELIN and SM22α in varicose vein tissue

doi: 10.3892/etm.2015.2170

Figure Lengend Snippet: Reverse transcription polymerase chain reaction analysis revealed weak mRNA expression of SM22α in the vascular smooth muscle cells (VSMCs) of the experimental group, but strong expression in the control group. (A) Expected length of the PCR products was 232 bp (SM22α) and 472 bp (GADPH). Lanes: N, control group; X, DNA marker DL 2000; and P, experimental group. (B) Downregulation of SM22α mRNA expression in the VSMCs from the varicose vein tissue. Data are presented as the mean ± standard deviation.

Article Snippet: TransZol Up and two-step reverse transcription polymerase chain reaction (RT-PCR) kits were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). β-actin upstream and downstream primers were synthesized by Takara Bio, Inc. (Shiga, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Marker, Standard Deviation

Journal: Frontiers in Immunology

Article Title: The roles of different forms of IL-15 in human melanoma progression

doi: 10.3389/fimmu.2023.1183668

Figure Lengend Snippet: Modulation of the surface expression of the mbIL-15 by PMA. (A) Surface expression of mbIL-15 before and after treatment with PMA at 100 ng/mL for 3 h was measured by flow cytometry on primary/metastatic pairs each derived from a single patient (WM-115/WM-266–4 and T1/G1) or on metastatic cell lines (A2058 and SK-MEL-28). Bar graphs show the MFI expressed as a percentage of the untreated controls. The results are the means ± SD ( n = 3); p -values were calculated using a Student’s t-test (* p < 0.05). mbIL-15, membrane-bound IL-15 complex; MFI, mean fluorescence intensity; PMA, phorbol-12-myristate-13-acetate. (B) RT-PCR analysis of ADAM17 mRNA was performed on the abovementioned samples before and after treatment with PMA. ADAM17 mRNA levels were normalized with GAPDH and were expressed as fold increases relative to the untreated controls. The results are the means ± SD ( n = 3); p -values were calculated using a Student’s t-test (* p < 0.05). ADAM17, disintegrin and metalloproteinase-17; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PMA, phorbol-12-myristate-13-acetate; RT-PCR, reverse transcription PCR. (C) Analysis of the public dataset TCGA concerning the correlation between ADAM17 expression and OS in melanoma stages I–IV. ADAM17, disintegrin and metalloproteinase-17; OS, overall survival; TCGA, The Cancer Genome Atlas.

Article Snippet: All cell lines were confirmed as mycoplasma-negative by reverse transcription PCR (RT-PCR) (Eurofins, Luxembourg).

Techniques: Expressing, Flow Cytometry, Derivative Assay, Membrane, Fluorescence, Reverse Transcription Polymerase Chain Reaction, Reverse Transcription